Biotin Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody,

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產品名稱: Biotin Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody,

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Biotin Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM103

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Product Details
Product Name	Biotin Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody [RM103]
Product Description	Biotinylated Rabbit monoclonal to Mouse Ig Kappa Light Chain
Catalog No.	31-1007-02
Clone Name	RM103
Product Data Sheet	Biotin Anti-Mouse Ig Kappa Rabbit Monoclonal Antibody Data Sheet RM103
Specificity	This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The Fc region of RM103 has been engineered to eliminate Fc receptor binding.
Immunogen	Mouse IgG
Application	Western Blot (WB) (nonreduced) Immunoprecipitation (IP) Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	Mouse
Conjugate	Biotin
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 0.005ug/mL-0.2ug/mL; Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL; Western Blot (WB): 0.1ug/mL-0.5ug/mL.
Keywords
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Product Images
	Western blot of nonreduced(-) and reduced(+)mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mLof RevMAb clone RM103. This antibody reacts to nonreduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa).
	ELISA of mouse immunoglobulins shows RM103reacts to the kappa light chain of mouse immuno-globulins. No cross reactivity with the lamda lightchain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immuno-globulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL ofRM103 was used as the primary antibody. Analkaline phosphatase conjugated anti-rabbitIgG as the secondary antibody.
	A titer ELISA of mouse IgG1κ. The plate was coatedwith different amounts of mouse IgG1κ. A serial dilution of RM103 was used as the primary antibody.An alkaline phosphatase conjugated anti-rabbitIgG as the secondary antibody.