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Biotin Anti-Human IgD Rabbit Monoclonal Antibody, Clone RM123

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產品名稱: Biotin Anti-Human IgD Rabbit Monoclonal Antibody, Clone RM123
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Biotin Anti-Human IgD Rabbit Monoclonal Antibody, Clone RM123


Biotin Anti-Human IgD Rabbit Monoclonal Antibody, Clone RM123  的詳細介紹

Product Details
Product NameBiotin Anti-Human IgD Rabbit Monoclonal Antibody [RM123]
Product DescriptionBiotinylated Rabbit monoclonal to Human IgD
Catalog No.31-1025-02
Clone NameRM123
Product Data SheetBiotin Anti-Human IgD Rabbit Monoclonal Antibody Data Sheet RM123
SpecificityRM123 reacts to human IgD. No cross reactivity with human IgG, IgM, IgE, or IgA.
ImmunogenHuman IgD
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateBiotin
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size50 ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 25ng/well – 200ng/well (for Capture);
0.01ug/mL – 0.1ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
.
Product Images
Immunohistochemistry

Immunohistochemistry of Human Lymphoid Tissue using
Anti-Human IgD antibody RM123.

Sandwich

Sandwich ELISA using RM123 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

ELISA

ELISA of human immunoglobulins shows RM123
reacts to IgD from human plasma and IgDκ from
human myeloma. No cross reactivity with human
IgG, IgM, IgA, or IgE. The plate was coated with
50 ng/well of different immunoglobulins. 200 ng/mL,
50ng/mL, or 10 ng/mL of RM123 was used as the
primary antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.

A

A titer ELISA using RM123. The plate was coated
with different amounts of human IgD (from plasma).
A serial dilution of RM123 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.


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