Biotin Anti-Human IgA2 Rabbit Monoclonal Antibody, Clone RM125
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產品名稱: Biotin Anti-Human IgA2 Rabbit Monoclonal Antibody, Clone RM125
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Biotin Anti-Human IgA2 Rabbit Monoclonal Antibody, Clone RM125
Biotin Anti-Human IgA2 Rabbit Monoclonal Antibody, Clone RM125
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Product Details |
Product Name | Biotin Anti-Human IgA2 Rabbit Monoclonal Antibody [RM125] |
Product Description | Biotinylated Rabbit monoclonal to Human IgA2 |
Catalog No. | 31-1027-02 |
Clone Name | RM125 |
Product Data Sheet | Biotin Anti-Human IgA2 Rabbit Monoclonal Antibody Data Sheet RM125 |
Specificity | RM125 reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE. |
Immunogen | Human IgA2 |
Application | Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA |
Species Reactivity | Human |
Conjugate | Biotin |
Intended Use | Research Use Only |
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Properties |
Form | Liquid |
Storage Instructions | Store at -20.0°C |
Stability | Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Volume/Item Size | 50 ug |
Concentration | 1.0 mg/mL |
Purity | Protein A affinity purified from an animal origin–free culture supernatant |
Clone Type | Monoclonal |
Isotype | Rabbit IgG |
Usage | ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.1ug/mL-1ug/mL.
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Product Images |
| Immunohistochemistry of Human Tonsil Tissue using
Anti-Human IgA2 antibody RM125.
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| Sandwich ELISA using RM125 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
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| Sandwich ELISA using RM125 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
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| ELISA of human immunoglobulins shows RM125
reacts only to Human IgA2. No cross reactivity
with Human IgA1, IgG, IgM, IgD, or IgE.
The plate was coated with 50 ng/well of different
immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM125 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
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| A titer ELISA using RM125. The plate was coated
with different amounts of human IgA2. A serial dilution
of RM125 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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