Biotin Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone RM108

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產品名稱: Biotin Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone RM108

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Biotin Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone RM108

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Product Details
Product Name	Biotin Anti-Mouse IgG2b Rabbit Monoclonal Antibody [RM108]
Product Description	Biotinylated Rabbit monoclonal to Mouse IgG2b
Catalog No.	31-1004-02
Clone Name	RM108
Product Data Sheet	Biotin Anti-Mouse IgG2b Rabbit Monoclonal Antibody Data Sheet RM108
Specificity	RM108 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The Fc region of RM108 has been engineered to eliminate Fc receptor binding.
Immunogen	Mouse IgG2b
Application	Western Blot (WB) (stronger on nonreduced) Immunoprecipitation (IP) Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	Mouse
Conjugate	Biotin
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	50ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 0.005 ug/mL – 0.2 ug/mL; Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL; Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL; Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images
	Western blot of nonreduced(-) and reduced(+)mouse IgG2b (20ng/lane), using 0.2ug/mL ofRevMAb clone RM108. This antibody reacts to nonreduced IgG2b (~150 kDa) much strongerthan the reduced γ2b form (~50 kDa).
	ELISA of mouse immunoglobulins shows RM108reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosph-atase conjugated anti-rabbit IgG as the secondary antibody.
	A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.