Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone RM219
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產品名稱: Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone RM219
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Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone RM219
Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone RM219
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Product Details |
Product Name | Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody [RM219] |
Product Description | Biotinylated Rabbit monoclonal to Mouse IgG2a |
Catalog No. | 31-1098-02 |
Clone Name | RM219 |
Product Data Sheet | Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody Data Sheet RM219
|
Specificity | RM219 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. |
Immunogen | Mouse IgG2a |
Application | Western Blot (WB) (nonreduced only)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
|
Species Reactivity | Mouse |
Conjugate | Biotin |
Intended Use | Research Use Only |
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Properties |
Form | Liquid |
Storage Instructions | Store at -20.0°C |
Stability | Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Volume/Item Size | 50ug |
Concentration | 1.0 mg/mL |
Purity | Protein A affinity purified from an animal origin–free culture supernatant |
Clone Type | Monoclonal |
Isotype | Rabbit IgG |
Usage | ELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.5 ug/mL-2 ug/mL. |
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Product Images |
| ELISA of mouse immunoglobulins shows RM219
reacts to the Fc region of mouse IgG2a; no cross
reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,
IgE, human IgG, or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins. 200
ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used
as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody. |
| A titer ELISA of mouse IgG2a. The plate was
coated with different amounts of mouse IgG2a. A
serial dilution of RM219 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
|
| Western blot of nonreduced(-) and reduced(+)
mouse IgG2a, using 0.5ug/mL of RevMAb clone
RM219. This antibody reacts to nonreduced
IgG2a (~150 kDa). |