Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121

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產品名稱: Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121

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Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121

Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121 的詳細介紹

Product Details
Product Name	Biotin Anti-Human IgM Rabbit Monoclonal Antibody [RM121]
Product Description	Biotinylated Rabbit monoclonal to Human IgM
Catalog No.	31-1023-02
Clone Name	RM121
Product Data Sheet	Biotin Anti-Human IgM Rabbit Monoclonal Antibody Data Sheet RM121
Specificity	RM121 reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
Immunogen	Human IgM
Application	Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	Human
Conjugate	Biotin
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
	Sandwich ELISA using RM121 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
	Sandwich ELISA using RM121 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
	ELISA of human immunoglobulins shows RM121 reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
	A titer ELISA using RM121. The plate was coated with different amounts of human IgM. A serial dilution of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.