Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117

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產品名稱: Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117

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Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117

Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117 的詳細介紹

Product Details
Product Name	Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody [RM117]
Product Description	Biotinylated Rabbit monoclonal to Human IgG1
Catalog No.	31-1019-02
Clone Name	RM117
Product Data Sheet	Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody Data Sheet RM117
Specificity	RM117 reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen	Peptide corresponding to the hinge region of Human IgG1
Application	Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	Human
Conjugate	Biotin
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
	Sandwich ELISA using RM117 as the capture antibody, and Biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
	Sandwich ELISA using RM117 as the capture antibody, and Biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
	ELISA of human immunoglobulins shows RM117 only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
	A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.