Biotin Anti-Human IgE Rabbit Monoclonal Antibody, Clone RM122

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產品名稱: Biotin Anti-Human IgE Rabbit Monoclonal Antibody, Clone RM122

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Biotin Anti-Human IgE Rabbit Monoclonal Antibody, Clone RM122

Biotin Anti-Human IgE Rabbit Monoclonal Antibody, Clone RM122 的詳細介紹

Product Details
Product Name	Biotin Anti-Human IgE Rabbit Monoclonal Antibody [RM122]
Product Description	Biotinylated Rabbit monoclonal to Human IgE
Catalog No.	31-1024-02
Clone Name	RM122
Product Data Sheet	Biotin Anti-Human IgE Rabbit Monoclonal Antibody Data Sheet RM122
Specificity	RM122 reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.
Immunogen	Human IgE
Application	Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	Human
Conjugate	Biotin
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 10ng/well – 100ng/well (for Capture); 0.01ug/mL – 0.1ug/mL (for Detection); Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
	Sandwich ELISA using RM122 as the capture antibody (25ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
	ELISA of human immunoglobulins shows RM122 reacts only to human IgEλ from human myeloma plasma and the IgE from human plasma. No cross reactivity with Human IgG, IgM, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL or 10 ng/mL of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
	A titer ELISA using RM122. The plate was coated with different amounts of human IgE. A serial dilution of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.