Product Details |
Product Name | Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody [RM120] |
Product Description | Biotinylated Rabbit monoclonal to Human IgG4 |
Catalog No. | 31-1022-02 |
Clone Name | RM120 |
Product Data Sheet | Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120
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Specificity | RM120 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
Immunogen | Peptide corresponding to the hinge region of Human IgG4 |
Application | Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Western Blot |
Species Reactivity | Human |
Conjugate | Biotin |
Intended Use | Research Use Only |
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Properties |
Form | Liquid |
Storage Instructions | Store at -20.0°C |
Stability | Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Volume/Item Size | 50 ug |
Concentration | 1.0 mg/mL |
Purity | Protein A affinity purified from an animal origin–free culture supernatant |
Clone Type | Monoclonal |
Isotype | Rabbit IgG |
Usage | ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Western Blot (WB): 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry (ICC): 1 ug/mL-10ug/mL;
Immunohistochemistry (IHC): 1 ug/mL-10ug/mL.
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Product Images |
| Sandwich ELISA using RM120 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
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| Sandwich ELISA using RM120 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
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| Western Blot of human, mouse, rat, and goat
IgG shows RM120 reacts to human IgG4, in
both whole molecule (~150kDa, non-reduced)
and heavy chain (~50kDa, reduced) forms. No
cross reactivity with other isotypes of human IgG,
or mouse, rat, or goat IgG.
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| A titer ELISA using RM120. The plate was coated
with different amounts of human IgG4. A serial dilution
of RM120 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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| Immunohistochemistry of Human Lymphoid Tissue using
Anti-Human IgG4 antibody RM120.
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