MicroVue Serum PYD EIA* Pyridinoline crosslinks (serum)

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產品名稱: MicroVue Serum PYD EIA* Pyridinoline crosslinks (serum)

產品型號: 8019

產品展商: Quidel

產品文檔: 無相關文檔

簡單介紹

MicroVue Serum PYD EIA* Pyridinoline crosslinks (serum)

MicroVue Serum PYD EIA* Pyridinoline crosslinks (serum) 的詳細介紹

Features & Benefits

Feature	Benefit

Product Specifications

Product Spec Name	Product Spec Data
Format	ELISA
Test/kit	96 wells/plate
Sample type	Serum

Performance Characteristics

Method: Competitive
Analyte: Pyridinoline
Specimen: Serum
Specimen Volume: 25 μL post-filtration
LOD*: 0.4 nmol/L
LLOQ*: 0.75 nmol/L
HLOQ*: 12 nmol/L
Precision CVs within-run: 6-15%
Precision CVs between-run: 9-12%
Incubation Time: 20-26 Hours
Cross-reactivity: Free PYD 100% Free DPD 0% PYD/DPD peptides < 2.5% L-arginine 0.0004% (see Package Insert)

*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification

About the Assay

Format

96 well microtiter plate, with reagents sufficient to test 40 samples in duplicate

Antibody Specificity

Free pyridinoline cross-links, some cross-reactivity to glutamine and arginine (0.5 nmol/L signal)

Species Reactivity

Baboon, Cat, Cow, Dog, Guinea Pig, Horse, Human, Rhesus Macaque, Mouse, Rat
No reactivity detected with Rabbit

Assay Steps

Spin serum in Spinfilters (included) for 30 minutes
Add 50 μL Reagent 1 to plate
Add 25 μL serum, diluted Standards, diluted Controls
Add 75 μL cold Pyridinoline Antibody
Incubate overnight at 2°C to 8°C
Wash 3 times with 1x Wash Buffer
Add 150 μL Enzyme Conjugate
Incubate 1 hour at room temperature
Wash 3 times with 1x Wash Buffer
Add 150 μL working Substrate Solution
Incubate 40 +/– 5 minutes at room temperature
Add 100 μL Stop Solution
Read at 405 nm
Data Reduction: 4 Parameter