MicroVue 25-OH Vitamin D EIA Total 25-OH Vitamin D

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產(chǎn)品名稱(chēng): MicroVue 25-OH Vitamin D EIA Total 25-OH Vitamin D

產(chǎn)品型號(hào): 8046 (CE)

產(chǎn)品展商: Quidel

產(chǎn)品文檔: 無(wú)相關(guān)文檔

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MicroVue 25-OH Vitamin D EIA Total 25-OH Vitamin D

MicroVue 25-OH Vitamin D EIA Total 25-OH Vitamin D 的詳細(xì)介紹

For In Vitro Diagnostic Use.

The MicroVue 25-OH Vitamin D EIA is an immunocapture assay for the determination of total 25-OH Vitamin D in human serum.

Vitamin D is a group of fat-soluble secosteroids which are produced in the skin of vertebrates after exposure to ultraviolet light from the sun, available from artificial sources, or occur naturally in a small range of foods. Total 25-OH Vitamin D is a combined measurement of two major forms, vitamin D₂ (ergocalciferol) and vitamin D₃ (cholecalciferol); known collectively as calciferol. When synthesized in the kidneys, calciferol circulates as a hormone; regulating, among other things, the concentration of calcium and phosphate in the bloodstream, promoting the healthy mineralization, growth and remodeling of bone.

Features & Benefits

Feature	Benefit

Product Specifications

Product Spec Name	Product Spec Data
Format	ELISA
Test/kit	96 wells/plate
Sample type	Serum

Performance Characteristics

Method: Immuno-capture
Analyte: 25-OH Vitamin D2 & D3
Specimen: Serum
Specimen Volume: 50 μL
Limit of Detection: 1.5 ng/mL
Dynamic Range: 0-133 ng/mL
Precision CVs within-run: 2.7%-5.7%
Precision CVs between-run: 4.3%-4.7%
Incubation Time: 3 Hours

About the Assay

Assay Steps

Add 50 μL of Standard, Control or sample
Add 150 μL of the Assay Buffer
Place on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 2 hours
Wash 3 times with 1X Wash Buffer
Add 200 μL of working HRP conjugate
Place on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 30 minutes
Wash 3 times with 1X Wash Buffer
Add 100 μL of the TMB Substrate
With appropriate cover to avoid light exposure, place the microplate(s) on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 15 minutes
Add 100 μL of Stop Solution and read at 450 nm
Data Reduction: 4 parameter logistics, or B/B0% interpolation