Format

96-well microplate with reagents sufficient to test 40 samples in duplicate Antibody Specificity: The antibody in the assay has a selective, high affinity for free Pyd and Dpd and negligible binding to Pyd and Dpd peptides.

Species Reactivity

Mouse, Rat, Guinea Pig, Rabbit, Pig, Dog, Sheep, Horse, Cynomolgus Monkey, Rhesus Macaque, Human Cell Culture

Assay Steps

• Add 50 μL of 1
• 10 diluted Standards, Controls and samples
• Add 100 μL cold Enzyme Conjugate
• Incubate 3 hours ± 10 minutes at 2°C to 8°C
• Wash 3 times with 1X Wash Buffer
• Add 150 μL room temperature Working Substrate Solution
• Incubate 60 ± 5 minutes at room temperature
• Add 100 μL Stop Solution and read OD at 405 nm
• Data Reduction: 4 Parameter
• Correct for creatinine concentration