Strain
AVB101, an E. coli B strain (hsdR, lon11, sulA1), contains pBirAcm , an
IPTG inducible plasmid containing the BirA gene engineered into
pACYC184. It is compatible with most cloning vectors and is maintained
with Chloramphenicol (10 μg/ml). This strain is recommended for protein
expression because of its robust growth and the absence of the OmpT and
Lon proteases.
At the time of induction, Biotin should be added at a concentration of 50 μM. The pAN and pAC vectors containing the AviTagTM require ampicillin (100 μg/ml).
Cells are supplied as a 0.5 ml liquid culture in 30% glycerol. Store at -80°C.
Note: AVB101 cells are not competent. For BirA expressing competent cells please look at related products.
