Strain
CVB101, an chemical competent E. coli B strain (hsdR, lon11, sulA1),
contains pBirAcm , an IPTG inducible plasmid containing the BirA gene
engineered into pACYC184. It is compatible with most cloning vectors and
is maintained with chloramphenicol (10 μg/ml). This strain is
recommended for protein expression because of its robust growth and the
absence of the OmpT and Lon proteases.
At the time of induction, Biotin should be added at a concentration of 50 μM. The pAN and pAC vectors containing the AviTagTM require Ampicillin (100 μg/ml).
Cells are supplied as 10 vials of 100μl liquid culture in 30% glycerol. Store at -80°C.
