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iLite? Type I IFN Assay Ready Cells (BM3049)

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產品名稱: iLite? Type I IFN Assay Ready Cells (BM3049)
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iLite? Type I IFN Assay Ready Cells (BM3049)


iLite? Type I IFN Assay Ready Cells (BM3049)  的詳細介紹

iLite? Type I IFN Assay Ready Cells

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iLite? Type I IFN Responsive cells are Type I IFN responsive reporter gene cells and can be used for measuring the bioactivity of Type I Interferon (IFN) or neutralizing antibodies (NAb’s) to Interferon beta or neutralizing antibodies to Interferon alpha.

iLite? Type I IFN Assay Ready Cells can measure the bioactivity of Type I Interferon or can indirectly measures NAb’s to human Interferon beta or NAb’s to Interferon alpha. The cells are growth arrested cells which are sensitive to human Type I Interferon. The iLite? IFN cells are the only commercially available cells capable of being used in a Reporter Gene Bioassay for either measuring Type I IFN or detecting neutralization of IFN beta activity or neutralization of IFN alpha activity.

References:

  1. Hermanrud C, Ryner M, Luft T, Jensen PE, Ingenhoven K, Rat D, Deisenhammer F, S?rensen PS, Pallardy M, Sikkema D, Bertotti E, Kramer D, Creeke P, Fogdell-Hahn A, on behalf of the ABIRISK consortium: Development and validation of cell-based luciferase reporter gene assays for measuring neutralizing anti-drug antibodies against interferon beta. J Immunol Methods 2016; 430: 1-9.
Product code
BM3049
Format
Assay Ready Cells
Tests
Cells can be used for measuring the levels of Type I Interferon (38 samples) or neutralizing antibodies to Type I Interferon (Interferon beta or Interferon alpha) (20 samples).
Shelf life
21 Months
Calculation
Semi-quantitative
Antibody class detected
Neutralizing antibodies to Type I Interferon
Antigen
Type I Interferon
Incubation time
Type I Interferon: 30min + 18 hours
NAbs to IFN alpha or beta 1a: 30min + 18 hours
Detection system
Luminescence
Availability
Research use only
  • Application
  • Background
  • Content

iLite? Type I IFN Assay Ready Cells can be used for measuring the bioactivity of Type I Interferon or neutralizing antibodies to Interferon beta or Neutralizing antibodies to Interferon alpha.

iLite? Type I IFN Assay Ready Cells are growth arrested cells which are sensitive to human Type I Interferon. The cells can be used in a number of different assays for measuring the bioactivity of Type I Interferon or NAb’s to IFN beta or NAb’s to IFN alpha.

Type I Interferons are produced by a wide variety of cells in response to viral infection and hence the presence of type I interferon is indicative of infection. IFN alpha is routinely used to treat chronic viral hepatitis and a wide variety of malignant diseases and IFN beta is routinely used to treat multiple sclerosis. The development of neutralizing antibodies (NAb’s) to either IFN alpha or IFN beta which correlates with a loss of treatment efficacy, is relatively common 1-2. The iLite? IFN cells are the only commercially available cells capable of measuring either the bioactivity of type I interferon, NAb’s to IFN beta or NAb’s to IFN alpha. 

iLite? Type I IFN Assay Ready Cells 1 vial (2.5mL)

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