MYC Monoclonal Antibody
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產(chǎn)品名稱: MYC Monoclonal Antibody
產(chǎn)品型號:
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簡單介紹
MYC Monoclonal Antibody
的詳細(xì)介紹
產(chǎn)品類型:Tag/Control Antibodies
產(chǎn)品名稱:
規(guī)格:
免疫原:
EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
宿主:
Mouse
應(yīng)用范圍:
ELISA,WB,IF,IP;Recommended dilution:WB:1:500-1:5000,IF:1:100-1:300,IP:1:1000-1:1500
背景:
Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein. It can help to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. Myc Tag also can be used to isolate protein complexes with multiple subunits. The Myc Tag Antibody is produced by the conjugation of a synthetic Myc tag peptide to KLH. Myc Tag Antibody can be used in various immunoassays, such as ELISA, Western blotting, immunoprecipitation, immunofluorescence, and more.
克隆類型:
Monoclonal
抗體亞型:
IgG1
純化方式:
>95%,Protein G purified
標(biāo)記方式:
Non-conjugated
保存緩沖液:
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式:
Liquid
儲存條件:
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
圖片:
WB:Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6ug/ml
Line 1 : Recombinant Myc-tagged fusion protein at 50ng
Line 2 : Recombinant Myc-tagged fusion protein at 25ng
Line 3 : Recombinant Myc-tagged fusion protein at 12.25ng
Line 4 : Recombinant Myc-tagged fusion protein at 6.25ng
Line 5 : Recombinant Myc-tagged fusion protein at 3.125ng
Line 6 : Recombinant Myc-tagged fusion protein at 1.5625ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size : 50kd
Observed band size : 50kd
Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunoprecipitating MYC-tag with transfected HEK293
Lane 1: Mouse control IgG(1μg)instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000041M0m(6μg)+ transfected HEK293 whole cell lysate(1mg)
Lane 3: Transfected HEK293 whole cell lysate (10μg)
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別名:
bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
